BIOL 354L - Cell and Molecular Biology Lab

1 Credits
2 Contact Hours
This laboratory course is designed to provide foundational laboratory experience for future biological laboratory science professionals. Course content introduces students to techniques commonly used in the cellular molecular laboratory including cell fractionation, cell growth and enzyme kinetics, DNA restriction enzyme analysis, and recombinant DNA technology including cloning and transformation. Laboratory exercises highlight the usage of instrumentation and project based research applications of various techniques in the study of cellular processes.

Grade Note(s): All BIOL prefixed courses required for the BS Biological Sciences degree program require a C or better grade for successful completion.
All BIOL prefixed courses taken as requirements in the Nursing programs require a C or better grade for successful completion.
Fees: Additional course fees apply.
Prerequisite(s): BIOL 336
Corequisite(s): BIOL 354
Course Learning Outcomes:
1. Evaluate advanced laboratory techniques.
2. Interpret data from assays and experiments.
3. Perform glutathione affinity column protein purification.
4. Perform western blotting techniques.
5. Perform advanced PCR reactions.
6. Construct DNA plasmids using cloning vectors.
7. Characterize recombinant clones.
8. Demonstrate proper usage of laboratory instrumentation.
9. Demonstrate ability to keep a thorough, neatly organized lab notebook.
10. Summarize computational analysis of DNA sequences.